刘杨

副研究员

学习&工作经历：

2022.10－至今       人工智能与数字经济广东省实验室（深圳）-副研究员(智慧医疗团队)

2020.07－2022.09  深圳华大生命科学研究院-生物学-博士后

2014.09－2020.06  北京科技大学-化学-理学博士

2010.09－2014.06  北京科技大学-材料化学专业-工学学士

邮箱：liuyang@gml.ac.cn

研究领域：基于微流控技术的肿瘤微环境研究；单细胞分析；功能细胞筛选

代表性成果：

1．Liu, Y.; Liu, Y.; Zheng, X.; Zhao, L.; Zhang, X. Recapitulating and Deciphering Tumor Microenvironment by Using 3D Printed Plastic Brick-Like Microfluidic Cell Patterning, Advanced Healthcare Materials, 2020, 9(6), 1901713.

2．Liu, Y.*; Wang, S.*; Lyu, M.*; Xie, R.; Guo, W.; He, Y.; Shi, X.; Wang, Y.; Qi, J.; Zhu, Q.; Zhang, H.; Luo, T.; Chen, H.; Zhu, Y.; Dong, X.; Li, Z.; Gu, Y.; Liu, L.; Xu, X.; Liu, Y. Droplet Microfluidics Enables Tracing of Target Cells at the Single-Cell Transcriptome Resolution, Bioengineering, 2022, 9(11), 674.

3．Xie, R.*; Liu, Y.*; Wang, S.; Shi, X.; Zhao, Z.; Liu, L.; Liu, Y.; Li, Z. Combinatorial Perturbation Sequencing on Single Cells Using Microwell-Based Droplet Random Pairing, Biosensors and Bioelectronics, 2022, 220, 114913.

4．Wang, S.*; Liu, Y.*; Li, Y.; Lyu, M.; Gao, K.; He, Y.; Wei, W.; Zhu, Y.; Dong, X.; Xu, X.; Li, Z.; Liu, L.; Liu, Y. High-Throughput Functional Screening of Antigen-Specific T Cells Based on Droplet Microfluidics at a Single-Cell Level, Analytical Chemistry, 2021, 94(2), 918-926.

5．Zhao, L.*; Liu, Y.*; Liu, Y.*; Zhang, M; Zhang, X. Microfluidic Control of Tumor and Stromal Cell Spheroids Pairing and Merging for Three-Dimensional Metastasis Study, Analytical Chemistry, 2020, 92(11), 7638-7645.

6．中国博士后科学基金会, 中国博士后科学基金, 2021M692212, 面向高通量纳米抗体筛选和研究的微流控平台开发, 2021-06 至 2022-08, 8万元, 结题, 主持。

发表论文论著：

1．Liu, Y.; Liu, Y.; Zheng, X.; Zhao, L.; Zhang, X. Recapitulating and Deciphering Tumor Microenvironment by Using 3D Printed Plastic Brick-Like Microfluidic Cell Patterning, Advanced Healthcare Materials, 2020, 9(6), 1901713.

2．Liu, Y.*; Wang, S.*; Lyu, M.*; Xie, R.; Guo, W.; He, Y.; Shi, X.; Wang, Y.; Qi, J.; Zhu, Q.; Zhang, H.; Luo, T.; Chen, H.; Zhu, Y.; Dong, X.; Li, Z.; Gu, Y.; Liu, L.; Xu, X.; Liu, Y. Droplet Microfluidics Enables Tracing of Target Cells at the Single-Cell Transcriptome Resolution, Bioengineering, 2022, 9(11), 674.

3．Xie, R.*; Liu, Y.*; Wang, S.; Shi, X.; Zhao, Z.; Liu, L.; Liu, Y.; Li, Z. Combinatorial Perturbation Sequencing on Single Cells Using Microwell-Based Droplet Random Pairing, Biosensors and Bioelectronics, 2022, 220, 114913.

4．Wang, S.*; Liu, Y.*; Li, Y.; Lyu, M.; Gao, K.; He, Y.; Wei, W.; Zhu, Y.; Dong, X.; Xu, X.; Li, Z.; Liu, L.; Liu, Y. High-Throughput Functional Screening of Antigen-Specific T Cells Based on Droplet Microfluidics at a Single-Cell Level, Analytical Chemistry, 2021, 94(2), 918-926.

5．Zhao, L.*; Liu, Y.*; Liu, Y.*; Zhang, M; Zhang, X. Microfluidic Control of Tumor and Stromal Cell Spheroids Pairing and Merging for Three-Dimensional Metastasis Study, Analytical Chemistry, 2020, 92(11), 7638-7645.

6．Lyu, M.*; Shi, X.*; Liu, Y.; Zhao, H.; Yuan, Y.; Xie, R.; Gu, Y.; Dong, Y.; Wang, M. Single-Cell Transcriptome Analysis of H5N1-HA-Stimulated Alpaca PBMCs. Biomolecules, 2023, 13(1), 60.

7．Lyu, M.*; Shi, X.*; Liu, X.*; Liu, Y.; Zhu, X.; Liao, L.; Zhao, H.; Sun, N.; Wang, S.; Chen, L.; Fan, L.; Xu, Q.; Zhu, Q.; Gao, K.; Chen, H.; Zhu, Y.; Li, Z.; Guo, W.; Zheng, Y.; Gu, Y.; Liu, L.; Wang, M.; Liu, Y. Generation and screening of antigen-specific nanobodies from mammalian cells expressing BCR repertoire library using droplet-based microfluidics. Analytical Chemistry, 2022, 94(22), 7970-7980.

8．Zhao, L.*; Xiu, J.*; Liu, Y.; Zhang, T.; Pan, W.; Zheng, X.; Zhang, X. A 3D Printed Hanging Drop Dripper for Tumor Spheroids Analysis Without Recovery. Scientific Reports, 2019, 9(1), 1-14.

9．Xiao, J.; Liu, Y.; Su, L.; Zhao, D.; Zhao, L.; Zhang, X. Microfluidic Chip-Based Wearable Colorimetric Sensor for Simple and Facile Detection of Sweat Glucose. Analytical Chemistry, 2019, 91(23), 14803-14807.

10．Zhao, L.*; Shi, M.*; Liu, Y.; Zheng, X.; Xiu, J.; Liu, Y.; Tian, L.; Wang, H.; Zhang, M.; Zhang, X. Systematic Analysis of Different Cell Spheroids with a Microfluidic Device Using Scanning Electrochemical Microscopy and Gene Expression Profiling. Analytical Chemistry, 2019, 91(7), 4307-4311.

11．Liu, C.; Chen, C.; Li, S.; Dong, H.; Dai, W.; Xu, T.; Liu, Y., Yang, F.; Zhang, X. Target-Triggered Catalytic Hairpin Assembly-Induced Core-Satellite Nanostructures for High-Sensitive “Off-to-On” SERS Detection of Intracellular Microrna. Analytical Chemistry, 2018, 90(17), 10591-10599.

12．Qiao, H.; Guo, T.; Zheng, Y.; Zhao, L.; Sun, Y.; Liu, Y., Xie, Y. A novel microporous oxidized bacterial cellulose/arginine composite and its effect on behavior of fibroblast/endothelial cell. Carbohydrate Polymers, 2018, 184, 323-332.

13．Cheng, Y.; Jiao, X.; Zhao, L.; Liu, Y.; Wang, F.; Wen, Y; Zhang, X. Wetting Transition in Nanochannels for Biomimetic Free-Blocking On-Demand Drug Transport. Journal of Materials Chemistry B, 2018, 6(39), 6269-6277.

14．Zhao, L.*; Guo, T.*; Wang, L.; Liu, Y.; Chen, G.; Zhou, H; Zhang, M. Tape-Assisted Photolithographic-Free Microfluidic Chip Cell Patterning for Tumor Metastasis Study. Analytical Chemistry, 2017, 90(1), 777-784.

申请专利：

1．赵亮，刘莹莹，刘杨，张学记。用于不同三维细胞团配对的装置及共培养方法，已授权，专利号：ZL201810503262.8

2．赵亮，刘杨，刘莹莹，潘文杰，张学记。一种基于3D打印技术的细胞图案化培养的装置及方法，实审中，专利申请号：CN201710118294.1

3．高开，刘龙奇，刘亚，吕孟华，孙娜，王诗雨，刘杨，陈琳喆，王媚娘，郑越，董宇亮，沈梦哲，甄志恒。一种抗原特异性抗体分泌细胞的检测和分选方法，实审中，专利申请号：CN202110820465.1

4．王诗雨，刘亚，吕孟华，高开，刘杨，陈琳喆。一种回收油包水液滴中内容物的方法及液滴生成装置，实审中，专利申请号：CN202110593330.6